IN VITRO SELECTION OF INTERSPECIFIC F1 TOMATO HYBRIDS SUITABLE FOR USE AS ROOTSTOCKS
Abstract
The aim – to investigate the influence of selective media in vitro on the viability and indicators of plant growth and development of regenerated plants of tomato interspecies hybrids F1 and to identify promising genotypes for use as rootstocks. Methods: General scientific, measuring, laboratory, statistical. Results. The effect of adding 50% culture fluid filtrate (CLF) of Alternaria solani (necrotrophic pathogen), 10 g/l of NaCl (salinity) and 0.1 g/l of hydroxyproline (drought) to nutrient media as selective factors, as well as a 1.5-fold increase in the content of the main nutrients according to the prescription of MS (medium II) on the viability and development of the vegetative mass and root system of test-tube plants of 8 interspecies hybrids of tomato F1 was determined. CLF of A. solani, NaCl and hydroxyproline caused a significant decrease in the viability of regenerating plants of the studied samples and inhibition of their growth and development. The most pronounced selective effect was exerted by hydroxyproline. Medium II did not significantly affect the studied parameters of plants, therefore it cannot be used for evaluation and selection of samples. Conclusions. The used approach makes it possible to evaluate and select in vitro strong tomato rootstocks with a developed root system, resistant to a complex of biotic and abiotic factors, among interspecific F1 hybrids. According to the complex of traits in isolated culture F1 hybrids BC-88 and BC-91 – T-5 / S. habrochaites, BC96 – K-7311 / S. habrochaites and BC-156 – S. pimpinellifolium / S. lycopersicum var. cerasiforme, which are promising for use as tomato rootstocks, were selected.
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