PRODUCTIVITY OF ROOT CELERY IN OPEN SOIL BY CULTIVATION WITH MERISTEMS IN CULTURE IN VITRO

  • T. V. Polishchuk Pavlo Tychyna Uman State Pedagogical University
  • V. V. Ketskalo V.V. Uman National University of Horticulture
Keywords: Сelery root, cultivar, nutrient medium MS (Murashige, Skoog), plant growth regulator, benzoaminopurine, yield

Abstract

Abstract. In recent years, the microclonal reproduction method of meristem in vitro in our country and beyond has received great development. The studies were performed at Uman National University of Horticulture. During 2016–2018, it was found that plant growth regulator were required to reproduce Anita and Healer root crops in in vitro culture using traditional MS agar medium (Murashige, Skoog). MS (Murashige, Skoog) nutrient medium was selected for control. To obtain the genetically identical material, the composition of the nutrient medium with a concentration of the plant growth regulator benzoylaminopurine (BAP) of 0.2%, 0.3, and 0.5% was investigated. It was noted that before planting from the culture vessels in the cassettes, the Healer plants formed more callus tissue than the Anita variety. It was found that application of MS + 6-BAP nutrient medium of 0.2% contributes to better growth of cultivated plants, seedlings, increased number of leaves and plant height, which significantly increases the yield of propagating material. Increasing the concentration of 6-BAP 0.3% led to a significant decrease in these indicators, and increasing to 0.5% did not contribute to plant growth. After planting the seedlings in open soil, the growth of the plants in the first stages was slow, and as they adapted to the growing conditions it accelerated. After 30 days after planting, the biometric indices of the plants were higher than their cultivation with the addition of 6-BAP 0.2 to the culture medium. A similar trend was observed 60 days after planting seedlings in open ground. Studies have shown that higher yields of investigated varieties and higher quality indicators of production were ensured by the cultivation of explants on MS + 6-BAP medium 0.2%. The use of MS (Murashige, Skoog) with a plant growth regulator benzoaminopurine (BAP) concentration of 0.2%, 0.3 and 0.5% was found to contribute to better seedlings growth, leaf growth and height, compared to controls plants, which significantly increases the yield of propagating material. However, it has been proved that for growing the yield of celery root crops Anita and Healer it is advisable to grow regenerating plants on MS + 6-BAP 0.2% nutrient medium. This makes it possible to additionally obtain 3.5–4.4 t/ha of valuable commodity products with a root diameter of 8.4–9.4 cm and a length of 6.2–7.2 cm.

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Published
2019-12-26
How to Cite
Polishchuk, T., & Ketskalo V.V., V. (2019). PRODUCTIVITY OF ROOT CELERY IN OPEN SOIL BY CULTIVATION WITH MERISTEMS IN CULTURE IN VITRO. Vegetable and Melon Growing, (66), 6-15. https://doi.org/10.32717/0131-0062-2019-66-06-15